FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661777
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55410.8372911116584589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.040.9642451
TGGTATC5950.040.220092
CCGCTAT400.00787281727.49852815
GTATCAA21850.025.8288141
AGTCCGG550.001225306825.0024454
AGAGTAC15750.024.7923211
GGTATCA11250.024.226171
CAGAGTA16300.023.9557710
TATCAAC24450.022.9504952
TCGACAT600.002036150522.91370818
CAACGCA26050.021.5311015
ATCAACG26350.021.2891833
AACGCAG26400.021.245656
GTACTAA650.00321808121.1767221
TCAACGC26600.021.0859094
CGAATTA804.4411363E-420.62389615
AGTACGG18950.020.46064413
GAGTACG19250.020.42747712
TCCGCGC700.004975386419.64180410
TACGGGT2105.456968E-1219.64180415
Produced by FastQC (version 0.11.5)