FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAGCCATATCGGGTTTGTCAGACATGGTTGCGGAGGAAAAGCAAAG11.8867924528301887No Hit
ACTAAGTAGTTATGCGAGTGGATAGATTGGTATGTAGAGGGAAAGAGGTC11.8867924528301887No Hit
GTTTTATCCCATCCAGCATGGCTGTGAAATCCTAAAGGTTCAAACCCCAG11.8867924528301887No Hit
CAATATGAACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTAT11.8867924528301887No Hit
GTCATGGATTTCAAGGTCTTTTAATCACCTTCGGTTTAATCTCTTTTTAA11.8867924528301887No Hit
ACATACGAATGCAAAAATACTAACAGAAACAAGTAAGAAACATAGTGATC11.8867924528301887No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTAAACAA11.8867924528301887No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.8867924528301887No Hit
ATCCATTCCATTGCATTCCATTCCATTCCATTCCCGTGCACTCGGGTTGA11.8867924528301887No Hit
GTATATTAAATAGCCATTTTTCCCAGTACCAAAGAACTAAAACCTCAGTC11.8867924528301887No Hit
CTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTTGTGCCTCAGCCTCCT11.8867924528301887No Hit
AGATAATCCACTTCCTGCCAGACTACACAGTTTTTCTTGCAGTCAAGACA11.8867924528301887No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACGTTTTTTTTTTT11.8867924528301887No Hit
GGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAGAA11.8867924528301887No Hit
TAACACAGCAAGACGAGAAGACCCTATGGAGAATTAATTTAATAATGCAA11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTTTGGCGT11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAACTCTCGTATTCCGT11.8867924528301887No Hit
ATACTGGACTTCCTTTGTTCCCTTTCTGCTCCCTGCCTCTTCCTTTTCTT11.8867924528301887No Hit
CATTTTAAGACAAGAGAAACCTAGAAGGTTGATTACAGCTTAATTTTTAT11.8867924528301887No Hit
GGTATCAACGCAGAGTACGGGAACAAGCCCATAAGCGTTCCCATTAAAGT11.8867924528301887No Hit
AACTTAAAGTCGAAATAATCAAAGAAAAATCTAAGATTGTAGTCAAGAAG11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATTCCGT11.8867924528301887No Hit
TATCAACGCAGAGTACGGGATAAAAATAATAAAATAAATAATTTTATAAT11.8867924528301887No Hit
CAGCTCCACGTGGTCGGGGTAGAAGCCTGTGGCCAGGCACACCAGTGTGG11.8867924528301887No Hit
GTATCAACGCAGAGTACGGGGTGCCTTCTCAGTCCCAACCCAGTACTCCC11.8867924528301887No Hit
CTTTTGTGCTTTTGTGTTTTTTATTTTTTTGTTTGTTTGTGTTTTTTTTT11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT11.8867924528301887No Hit
ACAGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAAAGA11.8867924528301887No Hit
GTAATCATCCAGATCTCAACGCAATTCAACGCTACCCCTGCAATACAAAA11.8867924528301887No Hit
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACAGGCAGAAAT11.8867924528301887No Hit
CATATACACATCTCCGAGCCCACGAGACCCTAAGACAAGGCCTTTGCCGA11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11.8867924528301887No Hit
ACCCAAAATCGAAGAATTATATAAATATATTATTAATAGAGCAAAAACAT11.8867924528301887No Hit
AAATGGAGATAATACCATCTTGCTGATAGGGTTGGTGTAGAGGTGAAAGA11.8867924528301887No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGA11.8867924528301887No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATATGGTATTCCGGCATGT11.8867924528301887No Hit
CTGGTGCGCCTGCCCGGGCACATCCTCCTGGACTCAATCATGGCTTGTGG11.8867924528301887No Hit
ACGCAGAGTCGACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAA11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTTTGGGGT11.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTTTGCCGT11.8867924528301887No Hit
ACCCAGATGGAATCCAATCAAATCCATCTTTATAATATGGAGAATAACTA11.8867924528301887No Hit
CTCCACCTCCCGGGTTCACACCATTCTCCTGCCTCAGTCTCCGGAGCAGC11.8867924528301887No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG11.8867924528301887No Hit
GAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTGGGCGCAGC11.8867924528301887No Hit
ATACAAATAAATTAAATTAAATTAAATTGAAATATAGTTGATTGAAGAGT11.8867924528301887No Hit
ATATGCATAGAGCCCAATTGGCAGGGATGGAGAGGGGACGGGGGAAGGTG11.8867924528301887No Hit
CCCAAGGGACCCGGAGGGGGCTGAGGATGAGTTTGTGACATCGATAGAGA11.8867924528301887No Hit
GTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAAT11.8867924528301887No Hit
CAATTTGAAGGGCCACAGGAAGTTATTTGCCTCTTTGAAGCGTTTTCCAA11.8867924528301887No Hit
GTAATAACACGGCACACCTGACCATGGCTAAGGCGTTTTTTGGTACTAAT11.8867924528301887No Hit
CTCCTGTCCTCTGTGCAGATCGAGGGCCACCCTGTCTCCCTGTCTCTTAT11.8867924528301887No Hit
ACTTTGTGCCAAGCATTGTTCTAGACACGGAGAGGAGGAAACTCCTGCCG11.8867924528301887No Hit
TTACTGCACCCAGTATCTACAGTAGTTTTGGTGATAAATCACAGTTGTTT11.8867924528301887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAGA50.085.07
AATCATC50.085.03
CATCCAG50.085.06
CCAGATC50.085.09
TCCAGAT50.085.08
TAATCAT50.085.02
ATCATCC50.085.04
TCATCCA50.085.05
GTAATCA50.085.01
Produced by FastQC (version 0.11.5)