FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611.320754716981133No Hit
GCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTTGCTTTTCCTCCGCAACCATAT11.8867924528301887No Hit
CTTTACAACAGACAACTATCCCTCAACATACAAAACTATACACTCGCATAACTACTAAGTC11.8867924528301887No Hit
ATTCTCAATATGTAGTTACTCAACATATTATAAAGATGGATTTGATTGATTTGATATAGTT11.8867924528301887No Hit
TAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT11.8867924528301887No Hit
AATTAGAAGGCAGATATGTCTAATGATGTCTCTATTTTTTGCTAGAGATGAGAGAAATGTA11.8867924528301887No Hit
GGATGAGGGATTTCTGGGCATGCTTGTCAGGCCAGGGAGTACTGGGTTGGGACTGAGAAGG11.8867924528301887No Hit
GTACGGGCAGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACCGCGTGAATTAAA11.8867924528301887No Hit
GAATCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
TTGGTATCAACGCAGAGTACGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCG11.8867924528301887No Hit
CTGCTGTACCTTCTGCAGTTCTTCAAATTTGGGGTCCTTCCGGGAAGTGGGTGGCAGGTAT11.8867924528301887No Hit
TACAAACAGTTACTTCGATCATAAAAAAAAAATAAAAAAACATACAACCAGCTAATGGATA11.8867924528301887No Hit
GGGGGGGGGGGCGCGGGGTGCGGTGGGGGGGCGGGGGGGGGGGGGGGCGGGGGGGGGGGGG11.8867924528301887No Hit
AACAGATCTACTTCCTTGTGTTCAACTTTACAAATTTTATTGTGGTGGTTCTTCTTGTGGC11.8867924528301887No Hit
CTGAAGAACCCCCCTATCAACACAAAGAGTCAGGCAGTGAAGGACCGGGCAGGCAGCATTG11.8867924528301887No Hit
GCACAAAGGTACGAAAGGTGTTGCAGCTTCATCGCATTCTTCAAATCTTCAATGGAACCTT11.8867924528301887No Hit
AACACAGTGCCAGAAGCCGCGGAAAAACAGAGGGTGCGAGCGATAAACGGATTTACAGGGC11.8867924528301887No Hit
GACCAGGAGAGCCGGAAGAGGTCAGCAGGCTGAGGGGGTGCTGTTAGCCTGTGTCACCGCC11.8867924528301887No Hit
AAGTACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA11.8867924528301887No Hit
GCACCAGACAAGGACATGAAACAACAGACTGTAAAAATGAGGTCTACTGGTCAAATTCTGA11.8867924528301887No Hit
ATACACACCAACAAAAAAAAAAAACACAAACAAACAAAAAAATAAAAAACAAAAAAGAAAA11.8867924528301887No Hit
GGAGACAGGGTGGCCCTCGATCTGCACAGAGGACAGGAGCTGTCTCTTATACACATCTGAC11.8867924528301887No Hit
GTCCCATGTTAACTGAAGGCAAATTCACTCAACCTCTCTAGTAAGGGACCCATGGGCCTAC11.8867924528301887No Hit
TTATTATTATATAATGTCTTTCTTTATCCCTGACAATATTCCTTGTTCAGAAGAATGCTTT11.8867924528301887No Hit
TAACAAAGCAGAGTACCGGGCCCCTAAATGCGTTTTTTCACTTATTCCCTTTTTCCCTATT11.8867924528301887No Hit
GAACACAGTGGTTCACGCCTGTAATCTCAGTATTTTGGGAGGCTGAGGCTGATGGATCACT11.8867924528301887No Hit
AGATTGAACGAAGACTCTACATATTTTTTAAATTTTTTTTATTTTTATAAAAAAAATATAA11.8867924528301887No Hit
GATAATATACTTTGTCCCCAGTAAAGAAGGCCAATTGCTCCTCACAATTATTTTTGGGATG11.8867924528301887No Hit
AAGTTGGGGCGAGCTATTGTGGTATTTCAGAGTTATGGAGGGAAATGAAGTTAAATTTGAA11.8867924528301887No Hit
ACACAGAACCTAGTCGGCATAAGGAGATTGTAGTTATACACATTCACAAAATATGATAACA11.8867924528301887No Hit
ATGCTTTAGGTAGTATTCCAAACAGGCATGAAACAACTGTGATTTATCACCAAAACTACTG11.8867924528301887No Hit
ATTGAGTATAGTAGTTCGCTTTGACTTGTTAAGTATTATCATGTACTGCTCGGAGGTTGGG11.8867924528301887No Hit
CACCAACACTAATATTGAACATATTGAATAAAGGGTGATTAGTACCAAAAAACGCCTTAGC11.8867924528301887No Hit
TCAAACGAATGGATTATAATCCAAAATGTTTCATTGTGGGAGAAGACAATGTAGGCTACAA11.8867924528301887No Hit
CTGCCCAACCTCTGGCTGTGGCACCTTGGACATGTTTCTTTCGATGTTTCAGCCTGCACTT11.8867924528301887No Hit
TGTTTGTTGGAGAAACTTGGATATGCAGAAAGGATAGAAGGAATTTAAGGAAAATAATAAA11.8867924528301887No Hit
GCCAGAGGGAACAACGGCAACTGGAACTGAAGGGCCAGGAGAAACTGGAGCAGCTGGAGCA11.8867924528301887No Hit
CCCGAGGTAGCTGTGTTTGAGCAATCAGAAGCAGAGATCTCACACACCCAAAAGGAAACAC11.8867924528301887No Hit
ATTATAAGCATGTGCCGCGGCCGACGAGCATACGGGGTTAGATCTCGGTGGTCGCCGTAGC11.8867924528301887Illumina RNA PCR Primer (95% over 23bp)
CATTTACAACTAAGCATTAGTGAAAAAAGAGGATTTAATACACCTGTCTCATATACAAATC11.8867924528301887No Hit
GAATCAAATAAAATTAAATGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA11.8867924528301887No Hit
AAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.8867924528301887No Hit
GTATTTGAGTTTTGGCTCTAAATCATCAATTAGGTGCCAGACATTTTAAAGTTTAACTTCT11.8867924528301887No Hit
CATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATAAATCTAAAAAAACGACATATA11.8867924528301887No Hit
GTGATATCAACGCAGAGTCGAGATATTATAAAAATATAAAAATTAATAATAACAATAGGGT11.8867924528301887No Hit
CTCTGATACGGATCTGCCGCTGCCGACGATAAGGGTCGTGTAGATCGCGGGGGTCGCCGGA11.8867924528301887No Hit
CATCAGCACTGAGCACCTACTTTGGGCCCTTTCCCGTGCTAGCCATTTGGGGGAAATATAG11.8867924528301887No Hit
TTTCTGATCACTATGTATCTTACTTCTTTCTGTTAGAATTTTTGCATTCATATATCTTTCT11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC50.055.04
GAGCGAT50.055.038
TAAACGG50.055.044
CGATAAA50.055.041
ACAGAGG50.055.027
TACAGGG50.055.054
AGAAGCC50.055.012
AAACAGA50.055.025
GCGATAA50.055.040
GAAGCCG50.055.013
AGAGGGT50.055.029
GGATTTA50.055.049
GAAAAAC50.055.022
TGCGAGC50.055.035
TTACAGG50.055.053
AGGGTGC50.055.031
GATAAAC50.055.042
CCAGAAG50.055.010
CGGATTT50.055.048
GCGGAAA50.055.019
Produced by FastQC (version 0.11.5)