FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923822
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48250.1650237257945251No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.12777795638722192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24150.049.280072
GTGGTAT27500.043.2798231
GGTATCA50000.040.806691
GTATCAA92750.038.8635141
TATCAAC105750.034.4051782
ATCAACG108250.033.4922373
TCAACGC109350.033.1936234
CAACGCA110900.032.653045
AACGCAG114950.031.465616
CGCAGAG124050.029.2228988
ACGCAGA125000.028.93337
GCAGAGT136900.026.5415489
CAGAGTA86500.025.08090610-11
GAGTACG92850.023.11428612-13
AGAGTAC79700.021.5153710-11
GTCGAGG13450.019.9062428
GTACGGG108550.019.59516714-15
GTACGAC2755.0222297E-918.546913
AGTACGG92450.018.47949612-13
TCGAGGT15200.017.3345159
Produced by FastQC (version 0.11.5)