FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008581
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.14562519510042163No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.12750294859903585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15900.042.5084532
GTGGTAT17200.040.0360341
GTATCAA55450.039.5560231
GGTATCA29600.038.342821
TATCAAC64200.034.364312
ATCAACG65000.033.614383
TCAACGC66100.032.9906774
CAACGCA66850.032.6841355
AACGCAG69200.031.6970586
CGCAGAG76650.028.6652958
ACGCAGA76250.028.6484577
GCAGAGT84900.026.2295449
TACCGTC1401.5327914E-624.2851037
CAGAGTA51550.023.45479410-11
GTATTAG9450.022.040871
GTCGAGG8000.021.78078
GAGTACG55800.021.28754812-13
AGAGTAC48900.020.29345510-11
CTATCGG1708.243887E-620.0034791
GTACGGG63500.018.37158414-15
Produced by FastQC (version 0.11.5)