FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008581
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130400.6492145449947003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20650.040.3584752
GTGGTAT21000.040.2378041
GTATCAA66350.023.8944361
GGTATCA37350.023.5079781
TATCAAC75850.020.814622
AGAGTAC51750.020.77692211
CAGAGTA53800.020.13857710
ATCAACG79700.019.7401243
TCAACGC80900.019.3409824
CAACGCA81450.019.2079895
TGCCGAC5050.019.06146855
AACGCAG82750.019.038196
CCGAATT3050.018.93270314
CGAATTA3100.018.62780215
ATAACCG909.830069E-418.3302965
AGTACGG59300.018.13072813
GAGTACG60200.017.81443812
TACGCGA950.001411987617.36683829
CGCAGAG91300.017.286298
ACGCAGA90750.017.2378487
Produced by FastQC (version 0.11.5)