FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176866
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26190.12031057492744156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16200.044.07912
GTGGTAT16350.043.4197271
GTATCAA56500.040.252581
TATCAAC62750.036.2391932
TCAACGC63350.035.9614074
CAACGCA64950.035.2063985
ATCAACG64500.035.189253
AACGCAG67250.034.0023166
GGTATCA28900.033.537091
ACGCAGA73550.031.0869567
CGCAGAG74900.030.5252428
GCAGAGT84500.027.0572859
CAGAGTA54050.022.60480710-11
AGAGTAC49500.021.33437210-11
GAGTACG60550.020.10801112-13
AGTCGAG11000.018.1586597
AGTACGG58200.017.99917412-13
GTACGGG69700.017.2853814-15
GTCGAGG7750.016.9988168
GACAGGT11050.016.9269521
Produced by FastQC (version 0.11.5)