FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176866
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149600.687226499012801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18300.038.205751
TGGTATC19150.036.343622
GTATCAA64600.025.736561
GGTATCA34100.023.2479271
TATCAAC70950.023.069652
AGAGTAC49550.022.97700311
TCAACGC73900.022.0284524
CAGAGTA52200.021.91691610
ATCAACG75050.021.7325443
CAACGCA75200.021.6456535
AACGCAG76650.021.1993316
ACGCAGA84550.019.2189967
AGTACGG59750.019.0541313
CGCAGAG85550.018.9656938
GAGTACG61200.018.55817612
GTATTAG5200.017.9978921
ACGGGTA2151.5097612E-1017.90508516
GCAGAGT91850.017.6955859
TACGGGT6200.017.29735415
GTACGGG68150.016.42272814
Produced by FastQC (version 0.11.5)