FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555399
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236220.9243957597228456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT26400.038.0433271
TGGTATC26200.037.370222
GTATCAA84550.025.0917131
GGTATCA46850.023.3168811
AGAGTAC62550.023.12559111
TATCAAC93600.022.5370182
CAGAGTA65950.021.93336910
ATCAACG97400.021.4276563
TCAACGC97500.021.4019094
CAACGCA98400.021.1773935
AACGCAG101750.020.4797526
TGCCGAC7800.020.09859555
AGTACGG72850.019.89369613
GTCGTAC1802.6739144E-1019.872861
GAGTACG74050.019.45990212
ACGGGAT12550.019.28143516
CGGGCTA1151.21285175E-519.12901117
CGCAGAG112950.018.4522518
ACGCAGA113550.018.3525947
TACGGGA40300.017.67376715
Produced by FastQC (version 0.11.5)