FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058863
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53530.17499966490817012No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46940.15345571213879144No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.10154099742289864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT30450.045.6503221
TGGTATC30800.044.713132
GTATCAA109200.039.823221
GGTATCA58450.037.891051
TATCAAC126850.034.2788282
TCAACGC129500.033.4783554
ATCAACG130300.033.3385933
CAACGCA131100.033.037355
AACGCAG134300.032.313466
ACGCAGA145400.029.8427097
CGCAGAG147950.029.2991498
GCAGAGT160500.027.1401069
CAGAGTA102000.023.60312710-11
AGAGTAC95800.021.58177410-11
GTCGAGG15450.021.1802988
GAGTACG114200.020.78389212-13
GTATTAG13800.020.330511
AGTACGG111650.018.47992912-13
GTACGGG130100.018.09696814-15
AGTCGAG23500.016.8186847
Produced by FastQC (version 0.11.5)