FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058863
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325751.0649381812784684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT36400.039.4659841
TGGTATC36950.038.1882172
GTATCAA130850.026.1006811
GGTATCA69100.023.9757541
AGAGTAC99150.023.85321811
TATCAAC147250.023.0694962
CAGAGTA103800.022.83800710
ATCAACG153950.021.9368423
TCAACGC154950.021.7906344
CAACGCA158050.021.4140225
AACGCAG158750.021.284966
AGTACGG115400.020.37518313
GAGTACG118100.019.95593812
ACGCAGA174850.019.3571457
CGCAGAG176000.019.2637988
GCAGAGT186600.018.2013519
GTACGGG133350.017.50849314
TACGGGG52050.017.11765115
TGCCGAC11900.016.87118355
TACGGGC15850.016.65566315
Produced by FastQC (version 0.11.5)