FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335432
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38130.16326743831548082No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.14965111379821808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25400.049.35942
GTGGTAT25500.048.5074921
GTATCAA80350.038.7240871
TATCAAC91900.034.6838572
TCAACGC94150.033.765424
CAACGCA94800.033.5787355
ATCAACG95400.033.2331923
AACGCAG98250.032.4861566
GGTATCA44500.030.4709971
CGCAGAG107050.029.8918448
ACGCAGA107250.029.8763647
GCAGAGT117450.027.2455569
CAGAGTA69950.023.72471410-11
GTCGTAC2002.6066118E-923.378611
AAGTACG4150.022.53361
AGAGTAC65850.021.94273610-11
GAGTACG81350.020.08724612-13
GTACGAC2602.5047484E-919.6150533
CCGCGAG1651.4864458E-418.028849
AGTACGG79300.018.00726112-13
Produced by FastQC (version 0.11.5)