FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335432
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221500.9484326668470757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25700.038.0212481
TGGTATC25600.037.7154352
GTATCAA94400.026.7962911
AGAGTAC71900.024.40028411
TATCAAC105750.023.8268722
TTAAACG3250.023.69069322
CAGAGTA74850.023.40187810
GGTATCA49200.023.329321
TCAACGC111300.022.48134
ATCAACG111950.022.4037323
CAACGCA112400.022.2852755
AACGCAG116200.021.5787726
AGTACGG83350.020.91639113
GAGTACG85350.020.39404112
TACGGGG36000.019.78422215
ACGCAGA127500.019.6451577
CGCAGAG127850.019.5500418
GCAGAGT133700.018.7156019
GTCGTAC2151.4733814E-1017.9234121
GTACGGG96200.017.83662214
Produced by FastQC (version 0.11.5)