FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864444
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59150.20649731675675978No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54440.1900543351519527No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.12086115141367748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC31700.047.9974142
GTGGTAT34450.044.047231
GTATCAA114200.040.271771
GGTATCA60550.037.27521
TATCAAC130900.035.9419862
TCAACGC137300.034.1433944
ATCAACG137650.034.1177333
CAACGCA139100.033.671015
AACGCAG143150.032.8371476
CGCAGAG156100.030.0286888
ACGCAGA157450.029.8262427
GCAGAGT167900.027.9684099
CAGAGTA105650.023.83300810-11
AGAGTAC99150.021.73077610-11
TAGGTCG1404.203291E-521.2506245
GTCGAGG15400.020.4208958
GAGTACG122600.020.41648912-13
ACGGGAT18150.019.5513816-17
GTACGGA9050.019.2563921
GTACGGG129900.018.69695514-15
Produced by FastQC (version 0.11.5)