FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325268
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284940.45047893622847285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT55750.035.2962531
TGGTATC56900.034.2275162
GTATCAA173700.024.4791361
GGTATCA97000.023.180231
AGAGTAC128850.022.06749711
TATCAAC197500.021.5326142
CAGAGTA137950.020.6915310
ATCAACG203850.020.6828423
TCAACGC207850.020.2677294
CAACGCA208500.020.201835
AACGCAG212700.019.8795346
TGCCGAC15100.019.30653255
AGTACGG149800.018.72399113
GAGTACG153400.018.33864412
ACGCAGA237450.017.8424597
CGCAGAG238900.017.7355658
GCAGAGT255800.016.6304239
TACGGGG68200.016.37011515
GTACGGG172700.016.01814714
CGATCAT8900.015.75935134
Produced by FastQC (version 0.11.5)