FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5709010
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT113280.19842319421405813No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT81080.14202112099996322No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68200.11946029171432525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC47550.044.424342
GTGGTAT47650.044.42421
GTATCAA155800.038.25021
GGTATCA88250.037.13581
TATCAAC178400.033.4969752
ATCAACG184650.032.1329963
TCAACGC185500.031.9854764
CAACGCA187950.031.4554635
AACGCAG195550.030.3850986
ACGCAGA214850.027.711317
CGCAGAG215350.027.5877658
GCAGAGT243400.024.4434039
CAGAGTA147200.022.086510-11
GTCGAGG25650.021.3725628
AGAGTAC135450.020.6452510-11
GAGTACG157500.020.53435912-13
AGTCGAG37500.018.4718597
GTATTAG25150.017.9151741
AGTACGG156650.017.70219812-13
GTACGGG178900.017.61519214-15
Produced by FastQC (version 0.11.5)