FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4813860
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT99230.20613395487197386No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74500.1547614596186844No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62890.13064359993851088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC43500.042.6968352
GTGGTAT44550.042.077361
GTATCAA146650.039.9705241
GGTATCA80800.038.03511
TATCAAC170350.034.5799672
TCAACGC170450.034.3352624
ATCAACG170700.034.3351333
CAACGCA172500.033.97655
AACGCAG175600.033.376696
ACGCAGA195050.030.0468717
CGCAGAG197100.029.7549928
GCAGAGT218550.026.8349119
CAGAGTA140100.023.60044110-11
GTCGAGG21700.022.1302388
GAGTACG148650.022.07157312-13
AGAGTAC130550.021.01348310-11
GTACGGG166900.019.27615714-15
AGTACGG143850.018.83437712-13
GTATTAG22100.018.0797821
TATACCG4059.094947E-1216.7906865
Produced by FastQC (version 0.11.5)