FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895995
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT161680.41499026564459146No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136500.350359792556202No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT134920.34630434587313386No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94370.24222310346907527No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.15788007941488633No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT54620.14019525179062087No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50260.12900427233607847No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT49500.12705355114675457No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC48490.12446114535567936No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA48290.12394779767427834No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC46560.11950734023015944No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC43070.11054942318971149No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.10469725962173977No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.10292621012090622No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA39720.10195084952624425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC65200.059.4544262
GTGGTAT77450.049.946731
GTATCAA278200.040.446781
GGTATCA161800.037.0185661
TATCAAC316600.036.194782
TCAACGC346600.033.0500954
ATCAACG348100.032.9203153
CAACGCA354300.032.3438155
AACGCAG356100.032.1564526
ACGCAGA383750.029.83537
CGCAGAG384400.029.8058158
GCAGAGT417250.027.4999499
GTCGAGG37100.027.2626158
CAGAGTA244350.025.77542910-11
ATACGGG6250.023.1252291
AGAGTAC240550.022.56085610-11
GACAGGG4900.022.5561181
GAGTACG282600.022.24245812-13
GACAGGT13150.021.9821571
ACGGGAT55750.021.26908916-17
Produced by FastQC (version 0.11.5)