FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125
Sequences flagged as poor quality0
Sequence length91
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA171.511111111111111No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT131.1555555555555554No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121.0666666666666667No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.8888888888888888No Hit
CATCAAATCCTTGATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGAT80.7111111111111111No Hit
CTTGATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTC60.5333333333333333No Hit
TCCTTGATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGT60.5333333333333333No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.5333333333333333No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4444444444444444No Hit
TTCCAACATCAAATCCTTGATGATTGCTCCATCGACTTCGTCTTCCTTAG50.4444444444444444No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT40.35555555555555557No Hit
GACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATG40.35555555555555557No Hit
CTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTCTCATTCCCCTG30.26666666666666666No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCTG30.26666666666666666No Hit
GAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATC30.26666666666666666No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGCTGTCTCTTATA30.26666666666666666No Hit
ATCAAATCCTTGATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATT30.26666666666666666No Hit
AAGGAAGACGAAGTCGATGGAGCAATCATCAAGGATTTGATGTTGGAATG30.26666666666666666No Hit
GATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTCTCA30.26666666666666666No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT30.26666666666666666RNA PCR Primer, Index 27 (95% over 23bp)
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26666666666666666No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC30.26666666666666666No Hit
TGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTCTCATT30.26666666666666666No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26666666666666666No Hit
CCATTCCAACATCAAATCCTTGATGATTGCTCCATCGACTTCGTCTTCCT30.26666666666666666No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.26666666666666666No Hit
GTATTACCTAGGTTTTCTTCTAGGGTTATTATTGTTTTAGGTCTTACATT20.17777777777777778No Hit
ATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTCTCAT20.17777777777777778No Hit
GTACGGGGCGAACGAGGGGAAGTGAATCTGTCTCTTATACACATCTCCGA20.17777777777777778No Hit
GCATAAGAGCGTGCAACTGCGACTGGGAATTCTTCTTCAAGATCATCCAC20.17777777777777778No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
GATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTC20.17777777777777778No Hit
CAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGC20.17777777777777778No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCCT20.17777777777777778No Hit
GGATTTGATGTTGGAATGGATAACCAAAAAAAAAAAAAAAAAAAAAAAAA20.17777777777777778No Hit
CGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT20.17777777777777778No Hit
GTGTTGGGCCCTTTAGTTTTCTTTTGGTTGTGTCGGATCTGTCTCTTATA20.17777777777777778No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
GTAGCAAAAACAATACAAAAAAACATAACAAGAACACAAAAAAAACTGTC20.17777777777777778No Hit
TTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCGTTCTCATTCCC20.17777777777777778No Hit
GTGGTAATAGCTGTATTCTTCTCTTTTAATTATCTCGTTATTTTTTGTAT20.17777777777777778No Hit
GTTTCACAGTTTTAACTTCAATTTTCTTGTTTACCCCACAAATGACAAAA20.17777777777777778No Hit
ATCCTTGATGATTGCTCCATCGACTTCGTCTTCCTTAGTGAGATTTTCCG20.17777777777777778No Hit
GTGAAGGTGACAGCAGTCGGTTGGAGCGAGCATCCCCCAAAAACTGTCTC20.17777777777777778No Hit
TATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
CTTCGGATTTGCTTCGGACAGGTTAATCTTCGCTTTTTCGCCATCTGGGT20.17777777777777778No Hit
GTCGATGGAGCAATCATCAAGGATTTGATGTTGGAACTGTCTCTTATACA20.17777777777777778No Hit
CCCCAAAATATTATAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17777777777777778No Hit
GAGTACGGGGGAAGGAAGGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17777777777777778No Hit
CAGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT20.17777777777777778No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACCTGTCTCTT20.17777777777777778No Hit
ACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGG20.17777777777777778No Hit
GATCTACACTCTTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA20.17777777777777778No Hit
CATTCCAACATCAAATCCTTGATGATTGCTCCATCGACTTCGTCTTCCTT20.17777777777777778No Hit
CACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGATTTGATGTTGG20.17777777777777778No Hit
GAGTACGGGGAGGCGTAGTCGATGGGAAATTGGTTAATATTCCAATACTT20.17777777777777778No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.17777777777777778No Hit
TATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTC20.17777777777777778No Hit
TATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGA20.17777777777777778No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCC20.17777777777777778No Hit
GGTATCAACGCAGAGTACGGGAAGGCGAAGAAAAGAATCTGTCTCTTATA20.17777777777777778No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)