FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878560
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT89710.47754663146239673No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC30290.16124052465718422No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.1381377225108594No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.11620603015075376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14000.049.798021
TGGTATC17000.041.0057642
GTATCAA51400.037.7963031
GGTATCA29600.037.4839251
ATCAACG60000.032.234563
TATCAAC60350.031.9763262
TCAACGC60900.031.6883854
CAACGCA61450.031.4047645
AACGCAG63800.030.3812566
ACGCAGA70550.027.4708167
CGCAGAG71150.027.35798
GCAGAGT76950.025.2944899
GTGTTAG6300.023.6169471
CAGAGTA46200.023.594710-11
GAGTACG49300.021.85245112-13
AGAGTAC41800.021.29986410-11
GTACGGG55800.019.11676214-15
CGTCAGA3950.018.2941975
AGTACGG49400.017.97994812-13
AGGGGGG24450.017.81615870-71
Produced by FastQC (version 0.11.5)