FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878560
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178540.9504088237799165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17750.038.7574121
TGGTATC17800.038.3179132
TAGCGCG705.7103625E-627.49944129
GTATCAA56800.023.2544481
GGTATCA32850.022.3660371
AGAGTAC41450.021.36151711
TATCAAC63650.020.783412
CAGAGTA43700.020.32567410
ATCAACG66650.019.718883
TCAACGC67350.019.4715524
CAACGCA67950.019.2991035
GAGTACG47250.018.7393612
AACGCAG70550.018.7037756
AGTACGG47750.018.60072513
TGCCGAC6350.018.1900655
ACGGGAT7500.017.23206516
AACGAAC1658.5889405E-716.66677351
CGCAGAG79000.016.6353578
ACGCAGA79050.016.622187
TACGGGA24850.016.59926215
Produced by FastQC (version 0.11.5)