FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444030
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168470.6893123243168047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23450.037.6710781
TGGTATC23450.036.9427342
GGTATCA42100.024.0553441
GTATCAA76650.024.019271
AGAGTAC55900.021.20183411
TATCAAC86600.021.1821122
ATCAACG88650.020.7190653
TCAACGC89200.020.4667534
CAACGCA89600.020.374555
CAGAGTA59250.020.23513410
AACGCAG91400.020.0025636
CGTTCTA1301.6380964E-619.03737610
AGTACGG63900.018.4613913
GAGTACG65300.018.06558612
ACGCAGA101850.017.9236357
CGCAGAG104200.017.4691418
CGGTACC2403.8198777E-1117.18863345
GCAGAGT110650.016.5267359
GTACGGG73050.016.07336214
TACGGGG28700.015.90501715
Produced by FastQC (version 0.11.5)