FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332417
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT160670.48214254098451664No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC49070.14725047915672018No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.14355946449679016No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41770.12534445719128187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29250.045.625731
TGGTATC29750.044.5718462
GTATCAA94850.036.743671
GGTATCA55700.034.8712121
TATCAAC106900.032.7996522
TCAACGC107550.032.7194824
ATCAACG108050.032.5685583
CAACGCA109850.032.111795
AACGCAG114150.030.9766086
CGCAGAG124050.028.4004178
ACGCAGA125100.028.2979267
GCAGAGT141450.024.9969649
CAGAGTA89350.022.07038510-11
TATACCG1754.3510045E-721.857025
AGAGTAC82050.020.4599710-11
GAGTACG96350.020.33460212-13
GTCGAGG15150.019.9164838
GTACGGG104350.018.26668414-15
AGTACGG92350.017.99394212-13
CGTCTTC47600.017.94619248-49
Produced by FastQC (version 0.11.5)