FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332417
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313230.9399483918129093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT33300.038.7646831
TGGTATC33550.037.1386682
GTATCAA110500.024.8336451
AGAGTAC80000.023.68348311
TATCAAC120050.022.8201142
CAGAGTA84550.022.4740210
GGTATCA60300.022.457191
ATCAACG125300.021.7977813
TCAACGC125250.021.7799454
CAACGCA126800.021.5120945
ATCGCGA650.003242016321.1527325
AGTACGG89400.021.0699213
AACGCAG132050.020.760946
CGGGATA4000.020.62329517
GAGTACG92500.020.42355512
TGCCGAC9850.019.82307655
TCGCGAC700.004979498719.64182326
CGCAGAG144250.018.872498
ACGCAGA145150.018.7925157
ACGGGAT14950.018.7609916
Produced by FastQC (version 0.11.5)