FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626801
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT114910.4374522470487867No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC35180.13392716083174933No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.11774778523382624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66300.039.105191
TGGTATC20750.036.45822
TATCAAC74950.034.760192
ATCAACG74850.034.693073
TCAACGC74750.034.6832854
GTGGTAT22900.034.336371
CAACGCA75900.034.213775
AACGCAG79500.032.6644746
GGTATCA35950.032.394391
CGCAGAG87350.029.7267078
ACGCAGA87250.029.7120697
GCAGAGT100450.026.0186889
CAGAGTA65900.022.02307310-11
GAGTACG67150.021.3281112-13
AGAGTAC60800.021.28415110-11
ACGGCGC1805.70697E-721.2498
GTATTAG10900.019.8866561
CTAGACT5400.019.67654
AGTACGG65900.019.28209712-13
GTCGAGG9800.018.6470818
Produced by FastQC (version 0.11.5)