FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626801
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229010.8718208954542046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23850.036.6727752
GTGGTAT25600.035.1595951
GTATCAA76400.026.3005941
AGAGTAC58200.023.24819611
TATCAAC85700.023.1397932
TCAACGC87950.022.3859064
ATCAACG88350.022.3182283
CAACGCA88900.022.2682915
CAGAGTA61150.022.21702410
GGTATCA39950.022.0971073
AACGCAG93100.021.4093346
AGTACGG65550.020.34735313
GAGTACG67050.019.97456412
ACGCAGA101900.019.479137
CGCAGAG102500.019.343818
ACGCGTA750.007418640418.33149733
GCAGAGT108900.018.309049
GTACGGG76250.017.45598414
TACGGGG31800.016.94992615
CAGTCGA1658.588013E-716.6675389
Produced by FastQC (version 0.11.5)