FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109211
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT147350.3585846528688841No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC50230.12223757796813062No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45560.11087286586159728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28900.043.240411
TGGTATC29400.041.203352
GTATCAA94950.037.110761
GGTATCA53750.033.7667851
TATCAAC104650.033.5080952
ATCAACG104700.033.167323
TCAACGC105500.032.996794
CAACGCA106400.032.677745
AACGCAG111000.031.3618186
ACGCAGA120750.028.8601327
CGCAGAG121650.028.5407688
GCAGAGT145600.023.9625059
CAGAGTA85200.022.29691710-11
AGAGTAC77400.020.39833510-11
GAGTACG93800.020.0263412-13
GTACGGG97550.018.97330714-15
AGTACGG84750.018.35369512-13
GTACGAC5450.017.9376833
GTATTAG14700.017.638141
GTCGAGG13500.017.6282838
Produced by FastQC (version 0.11.5)