FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109211
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300560.7314299509078507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32100.039.4379921
TGGTATC34500.036.1136442
GTATCAA107750.024.0599981
AGAGTAC80450.022.49120511
GGTATCA58350.022.120451
TATCAAC118700.021.7342342
CAGAGTA87350.021.25052310
ATCAACG122450.021.0216583
CAACGCA123650.020.8578115
TCAACGC124050.020.8139864
AACGCAG127450.020.278336
AGTACGG91300.019.75813513
ACGCAGA138300.018.6282567
GAGTACG97450.018.62408612
CGCAGAG141550.018.202328
TGCCGAC13350.017.30468855
GTACGGG103450.016.98548514
TACGGGG46800.016.86354815
GTACGAC3600.016.8062233
GCAGAGT158600.016.3854129
Produced by FastQC (version 0.11.5)