FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATGAAGCAGGGGATATTCCAACAGAATGGGAAGCTTGGATTGGAAG11.5151515151515151No Hit
AACTCACTCATTGGGCCAGAGACCCTTGCTGGCTGTCTCTTATACACATC11.5151515151515151No Hit
ATCTTAATATAAGCCTTTAATGTGCATTATCCGCTTGGTCAGTTGTGGTT11.5151515151515151No Hit
TATCAACGCAGAGTACGGGGTTAAAGAGTATCGTAAAGTTAAAGCGGCTG11.5151515151515151No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAAGGG11.5151515151515151No Hit
GTATGTTATGTTTGGTTTGTTTTGCTCAACTTAATGTTTTTGAGATTCAT11.5151515151515151No Hit
GGTGACTAGAGTTCAGGCGTGTGCTCTTCCGATCTCCCGTACTCCTGTCT11.5151515151515151TruSeq Adapter, Index 1 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
TCTCCAGCTTTTTCTCTTAGCTGAAGATTTGTCTCTTCTAGTTGTTTCTG11.5151515151515151No Hit
CAGCTACTCTGGAGGCTGAGGCAGGAGAATGGCGTGAACCTGGGAGGCGG11.5151515151515151No Hit
ACCTTGTGTGGCCTTGCATTCATCCCGTACTCTGCGTTGATACCCTGTCT11.5151515151515151No Hit
GTGGGAAAGATCAAGGTATCACTAGAGGTCAATGAAACAAAACATACAAT11.5151515151515151No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGGGAT11.5151515151515151No Hit
TTATATGTGCCCTTTCGTGTACGGGACTATCACCCTCTACGGTAGCACTT11.5151515151515151No Hit
GAAAAACATTAAAACCCTGATCACATGTTTGTAAGTAATCATTATGATTG11.5151515151515151No Hit
TCATGCCTCAGCCTCCTGAGTAGCTGGGACTATAGACTCAAGCTACCATG11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11.5151515151515151No Hit
CATTTAGCTTAAGAAGTTCTGAGTTATCTCCAAATCAACTCATTTCACTA11.5151515151515151No Hit
GATGAAGGGCCGAATCAGCTCTGGGTTCAACAGCGACTCCTCAAGAGGCC11.5151515151515151No Hit
TAAAATCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACA11.5151515151515151No Hit
GTTGTGTAGTGGGTTCGCTTATGCAAACGAGTATAATGACGATGCGGTCA11.5151515151515151No Hit
ATTTATTTCCATTCCATTGCAATCGAGCTGATTCCATTCCATTCCATTCC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCTGCATCGGGACTTTCGG11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT11.5151515151515151No Hit
ACTGTACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAA11.5151515151515151No Hit
AGCCGTTGCTTAGTGGTTCTCCTAGACATTTTAATTTTGAGAGTGTCACT11.5151515151515151No Hit
ACTTATACACATCAACGACCCCACGATACTAGGCATCAAAAAGAATTCCT11.5151515151515151No Hit
TAACTAGGGGTTGACAATGGTAAGTAACATAGTAGAAGTATCCTAGAACA11.5151515151515151No Hit
CAGTACTTAAATCATTTCTACACTGCAAAATCCAAATTATAAAAAATCTA11.5151515151515151No Hit
GAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAATTTTCACGCGGGGT11.5151515151515151No Hit
AGATTTCACTATGTCACCTAGTCTGGAGTGCAATGGTGCAATAATAGCTC11.5151515151515151No Hit
CACCATGACCAAAATCATAAGTATTGGTATTTAGTCTCAAATTTTTCCCA11.5151515151515151No Hit
CTCCTCCCAACTAGGCGCAGAGGGTAGCACAGACTTCCAAAAGGAAACAC11.5151515151515151No Hit
GTCAGAGATGGGCTGGTGCGTCACATTTGTCTTAGGGGGGTCCGCGCCCA11.5151515151515151No Hit
GATATAGCATGTTGAAGTTTGAGTTTTAGAATGATAATATGTAAACATAG11.5151515151515151No Hit
GAGTACGGGGATCACCTGAGGTCAGAAGTTTAAGACCAGCCTGGCCAACA11.5151515151515151No Hit
GAGATAAAACTATTGGATTTCGTTTCTTTTCTGTAGTCCTTTTTAAATAG11.5151515151515151No Hit
TTGCTATGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCTAGCAATCCTT11.5151515151515151No Hit
ATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTC11.5151515151515151No Hit
CTCCAGGAACGTAGAGATGAAGACAAACGTACTTCAGAATGGGCAGCAAG11.5151515151515151No Hit
CAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGA11.5151515151515151No Hit
GTATCAACGCAGAGTACGGGTAACTTGATTTTTGAGGTTAATATAATTCA11.5151515151515151No Hit
ATTCCAATACATTTCACTCCATTCCATTGCACTCGCGTTGAATCCATTCC11.5151515151515151No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11.5151515151515151No Hit
CTACCTACACTTGCGAGTTCATCGGCCAGTCAGTAGCATCAACTCCTGTC11.5151515151515151No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11.5151515151515151No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGGCG11.5151515151515151No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTTTTGGGGCCCC11.5151515151515151No Hit
TATCAACGCAGAGTACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
GCGTTATAATATTGTTAACATGGACATTATCTTGTAAAAATTTTTAATAT11.5151515151515151No Hit
CAATATCAAGTGGAGGTAAGTAGCTGGAGGAATCCTACTGTCTCTTATAC11.5151515151515151No Hit
CTGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGCATC11.5151515151515151No Hit
GTGTCGTTGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA11.5151515151515151No Hit
ATTATACACATCACCTACCCCACTAAAAAAGCACAGATATCCAAAACATA11.5151515151515151No Hit
GGATGGAGACCAGAGTCAAAGCACAAAACAAAGCTGGTCTGAACTGATGA11.5151515151515151No Hit
GTATGTATGCAGTAAGATAGCTTTAAATCCACATGAAACAAATGCAGATA11.5151515151515151No Hit
GATTCTACCACAGTCCAATGCTTCCCTCCTGGTTTAACATTTGGAGACTT11.5151515151515151No Hit
GTACTCTGCTGTGCGGTCATCTGTCTTCTGGGGGCAGGTCTCTCAAATGC11.5151515151515151No Hit
TCCCATGATGGTGCCCACTCGGCACGGAATGACTCGACCAGACAGATAAT11.5151515151515151No Hit
GGTTATAGGCATGAGCCACCGAGCCTGGGCACATTTATTTCTATATTCTT11.5151515151515151No Hit
AAACGAAACAGAAGCCCACAGCACAAAAAGAAGACCACAGACCTGTCTCT11.5151515151515151No Hit
CCTTTCCATTCAATTCCATTCCATTCAATTCAATTCCATTCAATTACAAT11.5151515151515151No Hit
GTACTGGCTTAAAGAATAAGGGGATGGAAAAAATGCATTATCTTCAGCAG11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCA50.085.07
TATACAC50.085.03
CATCACC50.085.09
TTATACA50.085.02
TACACAT50.085.05
ACACATC50.085.06
ACATCAC50.085.08
ATTATAC50.085.01
ATACACA50.085.04
AAAAAAA200.021.2560-61
Produced by FastQC (version 0.11.5)