FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441231
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT99980.4095474783009064No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC36530.14963762134759062No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.1323102975507029No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.1128938637924883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20600.037.971771
GGTATCA35450.037.8949281
GTATCAA63800.037.71431
TGGTATC20500.036.90672
TATCAAC72550.033.4532052
ATCAACG73800.032.7713973
TCAACGC74350.032.5289734
CAACGCA75700.031.9488645
AACGCAG77950.031.1902586
ACGCAGA86350.028.152087
CGCAGAG87150.027.8936568
GCAGAGT99750.024.3697479
CGTCAGA4350.022.4738585
GTCGAGG10250.021.9749978
CAGAGTA64050.021.63159810-11
AGCGTCA4550.021.4859983
TACCGTC1050.004188657320.237537
GAGTACG67350.020.22442212-13
AGAGTAC57500.019.84570710-11
GTACGGG71350.018.6438714-15
Produced by FastQC (version 0.11.5)