FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011912
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT83330.41418312530567936No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC31910.158605346555913No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30950.15383376608917287No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.12644688236861254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14850.044.651182
GTGGTAT15250.044.318361
GTATCAA55050.039.224981
GGTATCA30300.038.5785981
TATCAAC64200.033.5666282
ATCAACG67250.031.9178683
CAACGCA66900.031.8942475
TCAACGC67500.031.8626234
AACGCAG69600.030.840186
CGCAGAG77550.027.621068
ACGCAGA77950.027.588377
GCAGAGT86250.024.9322479
TATACCG1102.015206E-423.1842655
CAGAGTA54300.021.44473610-11
AGAGTAC48350.021.18315110-11
CGTACCC2301.3073077E-820.3282343
GTCGAGG10850.019.9770838
GAGTACG58250.019.66223312-13
GTATTAG8150.018.775921
TCGTACA1851.7106375E-518.3803182
Produced by FastQC (version 0.11.5)