FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541173
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158981.0315519412810892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16450.039.9818761
TGGTATC16400.038.9026452
GGTATCA32550.025.5321371
GTATCAA57600.024.8434581
AGAGTAC40750.023.15036811
TATCAAC64100.022.3948142
CAGAGTA43250.022.19375210
ATCAACG67850.020.9956343
TCAACGC68400.020.8220794
CAACGCA68550.020.775845
AACGCAG69900.020.4932566
ACCGGGT956.058038E-520.26458214
AGTACGG46900.020.17264713
TGCCGAC6000.019.70907855
TCACGCG700.004978505419.64168533
GAGTACG48250.019.60886812
ACGCAGA77800.018.2362127
CGCAGAG78350.018.109968
GCCGACG2950.017.71253455
GCAGAGT81250.017.568539
Produced by FastQC (version 0.11.5)