FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165302
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT38710.3321885657108629No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.14065023487473632No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.1214277500596412No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC13360.11464839157574604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9150.039.485881
TGGTATC9100.038.765322
GGTATCA17200.038.5513951
GTATCAA32100.036.943931
CAACGCA35900.033.0291825
TCAACGC36050.032.891754
ATCAACG36350.032.504773
TATCAAC36700.032.194782
AACGCAG37700.031.3394586
ACGCAGA40400.029.2437367
CGCAGAG40900.028.7823268
CCGGTGC1159.177764E-625.86848
GCAGAGT46650.025.2346659
CAGAGTA28600.023.55327410-11
GGGTACG1102.0142093E-423.1847551
ACGGGTA1303.6379788E-1222.88555116-17
TACGGGT3200.022.57904614-15
AGAGTAC26750.022.08406810-11
GAGTACG30650.021.90954212-13
GTATACG1000.00315336821.2526931
Produced by FastQC (version 0.11.5)