FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801441
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT29790.3717054655302137No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.18329484016914532No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.16071052017553383No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC10130.12639732681507435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7600.048.6736451
TGGTATC8000.043.0429422
GTATCAA28100.041.157671
TATCAAC32750.036.2159422
GGTATCA14800.035.9117471
ATCAACG33200.035.853113
TCAACGC33200.035.7250634
CAACGCA34700.034.4257775
AACGCAG35500.033.530236
CGCAGAG37250.031.830938
TCATACG550.004441304630.917493
ACGCAGA38800.030.6688637
TCCGGTC755.905471E-428.332198
GCAGAGT41950.028.264659
TACTAGA2200.027.0528052
ATACTAG1457.028393E-826.3914171
CAGAGTA26300.025.28890210-11
ACCGACG850.00122651924.9989937
CGTCAGA1452.0720327E-623.4546455
AGAGTAC24900.023.38259110-11
Produced by FastQC (version 0.11.5)