FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514199
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT767685.069875227760684No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT89030.5879676317313642TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC83830.5536260425479081No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71250.4705458133310087No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68580.4529127281156572No Hit
GAGTCGACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC67120.4432706665372253No Hit
GTGGTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTCCGAGCC53430.3528598288600111No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT49040.32386760260705494RNA PCR Primer, Index 10 (95% over 23bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.31448970709926505No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGT42660.28173311433966075No Hit
GAGTCGACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC37190.2456084041793714No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG32140.2122574377608227No Hit
GTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTCCGAGCCCAC29730.19634143200464405No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT28760.18993540479157628No Hit
ACGCAGAGTCGACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCC28360.1872937440851566No Hit
GAGTCGACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA28210.1863031213202492No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.18326521150786654No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT27430.18115188294273077No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26460.17474585572966303No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGCTCTCGTATGCCGT24630.1626602579977929No Hit
GGTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTCCGAGCCCA24520.16193380130352747No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.1565844383730276No Hit
GTGGTATCAACGCAGAGTCGACTCTGCGTCTGTCTCTTATACACATCTCC23370.15433902677257086No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.15354652856064493No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGC22780.15044257723060178No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18400.1215163924953061No Hit
GTATACACCATGAGCAAAGCTCACCCTCCCGAGTTGAAAAAATTTATGGA18270.1206578527657197No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC18180.12006347910677527No Hit
TGCCAGGACTGAGTTGTGGGGGAGGGAGGCGGTTAGCGGGCTTTAGCGCC17610.11629911260012718No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.1128649536817816No Hit
ACGCAGAGTCGACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA16630.10982704386939893No Hit
GGTATCAACGCAGAGTCGACTCTGCGTCTGTCTCTTATACACATCTCCGA15790.10427955638591757No Hit
CTCCATTCCATTGCACTCGGGTTGATTCCATTCCATTCCATTCCATTCCA15550.10269455996206577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT1050.076.911991
AAGTACG1200.074.3819961
TGGTATC42000.070.731782
GCTTAGT200.003222479763.7559931
GTGGTAT47050.063.326891
TCTACAC250.00779688450.999743
TCGCGGT250.00779688450.999742
AGTGGTA4700.049.7337882
ATACGGG901.2732926E-1147.226661
TGTTCGC901.2732926E-1147.221986
CGGTATC452.9272314E-547.221982
GCTAGTA452.9283741E-547.2188649
AGCGTCA1000.046.7497633
GTACTTG952.3646862E-1144.7410471
TGCTACG209.3235757E-442.49838318-19
TTCGGCC505.4540214E-542.498388
CAGCGTC1153.6379788E-1240.6519662
GTATTAG655.5594955E-639.234461
CTTATGG1207.2759576E-1238.9581382
CTGTGGT559.557979E-538.6399961
Produced by FastQC (version 0.11.5)