FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514199
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1472479.724415350954532No Hit
GAGTCGACTCTGCGTTGATACCACCTGTCTCTTATACACATCTGACGCTGCCGACGATATG55020.36336043016802944No Hit
GTGGTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATG51600.340774231128141No Hit
GAGTCGACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAG31480.20789869759523022No Hit
GTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAG27710.18300104543722456No Hit
GAGTCGACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA26200.1730287762704902No Hit
GTGGTATCAACGCAGAGTCGACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACG22650.14958403750101537No Hit
GGTATCAACGCAGAGTCGACTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA21600.14264967814666368No Hit
ACGCAGAGTCGACTCTGCGTTGATACCACCTGTCTCTTATACACATCTGACGCTGCCGACG20450.13505490361570704No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.1126668291288001No Hit
TGCCAGGACTGAGTTGTGGGGGAGGGAGGCGGTTAGCGGGCTTTAGCGCCTTTTCTGGCGG15890.1049399715625225No Hit
GTATACACCATGAGCAAAGCTCACCCTCCCGAGTTGAAAAAATTTATGGACAAGAAGTTAT15860.104741847009541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT150.005756603655.0659521
ACATCGG150.00578452854.99864249
TACGGTA150.005786040354.9950116
TGGTATC50000.047.595842
GTGGTAT51900.045.729141
AGTGGTA4650.040.8243262
TTAAACG1200.036.66576422
CGGAGAT604.1536623E-836.6657649
CAGACTA300.00192636136.66455510
TCAACGG300.001926673536.663345
GGCGAAG2550.032.35214623
GCGAAGA2600.031.72998824
AAGGCGA2600.031.72998821
GTATTAG350.004076061331.466261
ATCACGG350.004097221431.432993
CGAAACG350.004099872431.42883755
CGACCAT350.004101198631.42675852
TTGGCAG801.5041223E-830.9346925
GCTTATG1350.030.5921971
TATCACG453.786378E-430.5689412
Produced by FastQC (version 0.11.5)