FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020637
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT132290.6546945344463156No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.27590309392533147No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48010.2375983415130971No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC45350.22443417595540416No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.17623155470279916No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.1112025564215641No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.1010572408601842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24500.054.130442
GTGGTAT26000.051.012581
GGTATCA52150.043.0403371
GTATCAA101200.039.822071
TATCAAC116700.034.930182
ATCAACG122450.033.2205053
TCAACGC123350.032.97734
CAACGCA124600.032.612355
AACGCAG128300.031.7381156
CGCAGAG139200.029.2470848
ACGCAGA140100.029.120597
GCAGAGT146200.027.9637079
CAGAGTA87050.026.2163710-11
GTCGAGG16200.025.7079478
TGGACCG1251.74994E-523.8029255
GAGTACG97800.023.11744512-13
TCGAGGT17500.022.8274219
AGAGTAC83900.022.43927610-11
GCAGCGT4650.021.9408931
AGTCGAG22800.021.248937
Produced by FastQC (version 0.11.5)