FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020637
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275951.3656584532501383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28000.042.726912
GTGGTAT30050.039.9204181
TGCCGAC7600.026.05488455
GTATCAA109600.025.7817841
GGTATCA57900.025.7082041
AGAGTAC83000.023.29302811
CAGAGTA85200.022.85295710
TCCGCGC1103.2891148E-722.50027547
TATCAAC125500.022.2429582
GCGTTAC650.003231067421.1645831
ATCAACG133900.020.8023833
TCAACGC133950.020.7930744
CAACGCA135600.020.5172445
AACGCAG137900.020.2742216
CGATCGG956.0630984E-520.26290316
GAGTACG95950.020.0346112
AGTACGG96400.019.7699213
CGTGCGG700.00497625219.64358110
TAACGGC2500.018.70161636
CGCTAGG1051.3067602E-418.33446545
Produced by FastQC (version 0.11.5)