FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467052
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217420.8812947598996698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22350.038.7739142
GTGGTAT22950.038.61971
GTATCAA84500.026.2225481
GGTATCA43650.024.4671381
AGAGTAC60000.023.00588211
TATCAAC95000.022.877582
CAGAGTA62650.022.03276810
TCAACGC98400.021.9082364
ATCAACG99100.021.7570133
CAACGCA99350.021.724225
AACGCAG102600.021.3023226
AGTACGG66600.020.47829613
GAGTACG69000.020.00511412
ACGCAGA113250.019.2759557
ACGGGAT13450.019.21728516
CGCAGAG114850.018.9606938
GTACGGG75300.017.89281814
GCAGAGT122950.017.712999
TACGCGA1252.4869867E-517.59812529
TACGGGG28750.017.3115515
Produced by FastQC (version 0.11.5)