FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357742
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT80570.5934117085572959No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.19863862206516408No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC25910.19083154236961072No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.1658636176828882No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.15400569474907605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12850.054.2561531
TGGTATC13600.049.3794752
GGTATCA27300.040.6430321
GTATCAA49000.035.918031
TATCAAC57400.030.7301332
ATCAACG59200.029.580383
TCAACGC59200.029.580384
CAACGCA59550.029.335155
GTCGTAC1452.1791493E-929.3184011
AACGCAG60650.028.8731846
GTCGAGG7350.027.7545478
ACGCAGA67750.025.8445037
CGCAGAG68350.025.679818
GCAGAGT75200.023.4527919
CAGAGTA43350.023.43004410-11
AGTCGAG11100.023.3553897
AGAGTAC40950.021.68985610-11
TCTACAC2951.8189894E-1221.6121273
GAGTACG47650.021.2710912-13
GTATTAG6900.020.9477841
Produced by FastQC (version 0.11.5)