FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509654
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131930.8739088559365258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14000.034.1919021
TGGTATC13800.033.6772732
GGTATCA23800.027.0483591
GTATCAA45800.025.9489821
AGAGTAC34550.022.60474411
TATCAAC53200.022.0205042
CAGAGTA35850.021.86247810
TCAACGC55300.020.9805014
ATCAACG55200.020.919593
CAACGCA55750.020.8111535
AACGCAG56450.020.6484416
TTGCGTA700.004973154519.64530854
AGTACGG40200.019.35930313
GAGTACG41050.019.0924212
ACGCAGA63150.018.4141797
CGCAGAG63700.018.344548
GTCGTAC750.007396280318.3404831
GCTCGGT1352.3478242E-618.33318911
ACGGGAT6050.017.72595816
TACGGGA20250.017.38315615
Produced by FastQC (version 0.11.5)