FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657503
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT113520.6848856382160394No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.40313652524309157No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.3820807564149205No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.23849127271564516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC37730.22763156386443945No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25820.15577649029896173No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.1426845079616749No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.1370133266727119No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA21270.12832555959174735No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC20590.12422300291462521No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT20440.12331802717702471No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.11764684588806175No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.10835576164869687No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA17070.10298623893893405No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA16780.10123661917957312No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT16780.10123661917957312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28700.061.464282
GTGGTAT31950.055.0839921
GTATCAA126400.043.317631
GGTATCA61600.038.3698431
TATCAAC144950.038.1811832
TCAACGC153650.035.8245474
AACGCAG154700.035.636356
ATCAACG155550.035.3060463
CAACGCA156550.035.1337665
CGTCAGA1553.6379788E-1232.907365
CGCAGAG168550.032.6779068
ACGCAGA169400.032.539037
GCAGAGT175150.031.5426459
CAGAGTA108100.026.69411710-11
GACAGGC3300.025.763921
AAGTACG3350.025.3793851
GTCGAGG14950.024.7318178
GACAGGT7850.023.8275241
AGAGTAC106400.023.44595310-11
TCGAGGG1502.792698E-622.6654139
Produced by FastQC (version 0.11.5)