FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196592
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT86450.39356421219780463No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC31720.14440551545302904No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27040.12309978366487724No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.10684733441622295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15450.042.6408041
TGGTATC15800.039.5441672
GTATCAA50450.039.2596971
GGTATCA28600.034.775391
TATCAAC57700.034.4003832
ATCAACG58600.033.6544573
TCAACGC58950.033.5988434
CAACGCA60000.033.0108645
AACGCAG63000.031.5738476
ACGCAGA69100.028.7846077
CGCAGAG69050.028.68178
GCAGAGT81300.024.4112039
CAGAGTA46150.021.68761310-11
GTATTAG7600.021.2516271
AGAGTAC42200.020.69626210-11
GAGTACG49550.019.98548512-13
GTCGAGG9650.018.937558
AGTACGG46900.018.66795712-13
GTACGGG53950.018.07959614-15
TATATCG1450.001297463317.5875555
Produced by FastQC (version 0.11.5)