FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196592
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180700.8226379773758622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18750.037.583111
TGGTATC20050.034.7122762
GTATCAA60450.023.3601231
GGTATCA35150.022.3972531
AGAGTAC43950.021.0232111
TATCAAC66400.021.0047152
ATCAACG68500.020.2370723
TCAACGC69000.019.9672014
CAGAGTA46600.019.8871510
CAACGCA69900.019.7472065
AACGCAG71200.019.4621186
TGCCGAC7000.018.85868355
AGTACGG48850.018.74555613
GAGTACG51000.018.11706212
ACGCAGA77550.017.832657
GTATTAG6850.017.6813811
CGCAGAG78700.017.4347118
GTTCTAG4200.017.0403351
TCGGTAT1851.5808473E-716.34970729
GTACGGG55350.016.295414
Produced by FastQC (version 0.11.5)