FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395019
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT125790.525215040047699No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.19857880041870232No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.1782031791814595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC40620.16960199480672178No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30360.12676308622186297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22650.050.6699941
TGGTATC22650.050.102812
GTATCAA84800.037.694441
GGTATCA44850.037.151721
TATCAAC97200.033.5825732
ATCAACG99650.032.4583443
TCAACGC100150.032.29634
CAACGCA101150.031.9349825
AACGCAG103650.031.1647266
CGCAGAG116950.027.7261098
ACGCAGA116650.027.6886967
GCAGAGT124800.025.9815759
GTCGAGG14400.023.609798
CAGAGTA70150.023.14269310-11
AGAGTAC65500.021.89831410-11
AGTCGAG20700.021.5572137
GAGTACG76200.021.1381512-13
GTATTAG9500.019.239781
AGTACGG74700.019.00240712-13
GTACGGG85400.018.51300214-15
Produced by FastQC (version 0.11.5)