FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470002
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT154480.625424594797899No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52930.214291324460466No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC48720.19724680384874183No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45840.18558689426162409No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.12574888603329065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29150.049.5760271
TGGTATC29000.049.0956462
GTATCAA97400.040.3658521
GGTATCA50750.037.8559151
TATCAAC114400.034.7361532
TCAACGC118400.033.7434844
ATCAACG119750.033.363083
CAACGCA120000.033.1873175
AACGCAG124250.032.154766
CGCAGAG134950.029.5101688
ACGCAGA135400.029.474877
GCAGAGT143900.027.7327029
AGCGTCA3250.026.1553423
CAGAGTA86000.023.81968110-11
CTACGCT1652.4657675E-723.1831444
AGAGTAC81650.021.835510-11
GAGTACG94900.021.36188512-13
TAAGACG2006.6766006E-821.2512135
GTCGAGG14100.019.5929228
CTGATCG1100.005481212419.3181119
Produced by FastQC (version 0.11.5)