FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470002
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310471.2569625449695991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT37050.041.367311
TGGTATC36550.041.304152
GTATCAA118250.025.5034941
GGTATCA64700.023.970373
AGAGTAC81700.023.62830411
CAGAGTA84150.023.13644410
TATCAAC135700.022.0676822
GACCGCG650.003246781221.1471127
AGTACGG91700.020.78171713
ATCAACG143900.020.714213
TCAACGC144200.020.6105694
GAGTACG93700.020.51422312
CAACGCA145300.020.4343745
AACGCAG147700.020.1759686
CGGGATA4500.020.16596217
CCGAATT3950.019.49298914
ACGGGAT16300.018.89504416
CGAATTA3800.018.81551215
TGCCGAC9350.018.5321455
CGCAGAG164350.018.1360178
Produced by FastQC (version 0.11.5)