FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231160
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214040.9593216084906505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20300.039.8450622
GTGGTAT22650.037.065441
CGTACGA401.9012252E-434.37774335
GGTATCA38900.026.1812061
GTATCAA76750.025.8221071
TCCGACG550.001227628324.99751331
AGAGTAC54600.023.52030611
TATCAAC85200.023.1204682
CCGACGC600.002037304522.91438932
CAGAGTA57800.022.40844710
ATCAACG89600.021.824223
TCAACGC89800.021.7420624
CAACGCA91250.021.4233475
ACGTACG650.003240878221.15363934
AACGCAG93900.020.9930216
AGTACGG62100.020.5025613
GAGTACG63800.020.0855612
TGCCGAC7400.019.69663655
TACGGGT5950.019.4120115
GTACGAC1008.98585E-519.251973
Produced by FastQC (version 0.11.5)