FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934076
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT102500.1727311884782062No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.15232363050287864No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76840.12948940997722308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC51100.044.9154362
GTGGTAT53050.043.8289761
GTATCAA164350.037.63161
GGTATCA92700.035.3077741
TATCAAC192550.032.6473122
TCAACGC193400.032.4598734
ATCAACG196250.032.096773
CAACGCA195650.032.086585
AACGCAG206200.030.568586
ACGCAGA222800.028.1923187
CGCAGAG224950.027.9216888
GCAGAGT250850.025.140459
CAGAGTA156650.021.56773810-11
GCGTCAG12350.020.993544
GAGTACG161950.020.42909812-13
AGAGTAC144350.020.41726310-11
GTCGAGG26400.020.2824258
AGCGTCA13300.019.8135743
GTACGGG171800.019.07252714-15
AGTACGG152900.019.03946112-13
Produced by FastQC (version 0.11.5)