FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934076
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG453030.7634381494271392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT58150.039.1340071
TGGTATC57800.038.590712
GTATCAA193850.025.4088941
GGTATCA107500.023.1141431
AGAGTAC143850.022.69119511
TATCAAC221950.022.08222
CAGAGTA153750.021.51663610
TCAACGC228450.021.2792534
ATCAACG230750.021.1061023
CAACGCA231850.021.0491525
TACGCGA1701.2551027E-1021.02898429
AGTACGG156150.020.74530613
AACGCAG239350.020.5039316
GAGTACG162600.020.04076612
TACGGGG67400.018.60449215
TGCCGAC15700.018.56869355
ACGCAGA264050.018.5340277
GTACGGG172900.018.52849414
CGCAGAG267200.018.2035738
TACGGGA78450.017.45618215
Produced by FastQC (version 0.11.5)